What is ISO 19344?

ISO 19344 is officially titled:

“Milk and milk products — Starter cultures, probiotics and fermented products — Quantification of lactic acid bacteria by flow cytometry”

This standard specifies a flow cytometry-based method to quantify live (viable) and dead lactic acid bacteria (LAB) cells in dairy starter cultures, probiotics, and fermented milk products.

It includes:

  1. ✅ Guidelines for sample preparation, staining, and data acquisition
  2. 📊 Analysis methods to distinguish between intact (live) and damaged (dead) cells
  3. 🔬 Quantification using flow cytometry, enabling high-throughput and real-time analysis

This method is applicable to both industrial fermentation processes and quality control in final products

Why is ISO 19344 important?

Traditional plate-counting methods for LAB are time-consuming, less sensitive, and often fail to account for viable but non-culturable (VBNC) cells. ISO 19344 addresses these issues by introducing a modern, flow cytometry-based approach that is:

  1. 🔬 Faster and more accurate
  2. 💡 Able to detect non-culturable but viable bacterial populations
  3. 📈 Suited for high-throughput industrial applications

As demand rises for functional foods, probiotics, and precision fermentation, ISO 19344 plays a vital role in ensuring product efficacy and labeling accuracy.

What are the benefits of ISO 19344?

Implementing ISO 19344 enables dairy and probiotic manufacturers to:

  1. Accurately quantify viable lactic acid bacteria

           Crucial for product effectiveness, especially in probiotic claims.

  1. ⏱️ Reduce testing time

           Flow cytometry yields result in minutes to hours, unlike traditional methods that take days.

  1. 📦 Ensure batch-to-batch consistency

            Maintain microbial quality across production cycles.

  1. 🔬 Improve R&D efficiency

           Support innovation in starter cultures and functional dairy formulations.

  1. 🌍 Meet global quality and safety requirements

          Recognized by international regulatory bodies and food testing authorities.


What kind of businesses can benefit from ISO 19344?

Adopting ISO 19344 through GMC’s consulting and testing support offers businesses a strategic edge:

  1. Enhance Product Label Accuracy

           Meet probiotic CFU (colony-forming unit) claims confidently with validated results.

  1. Improve Quality Assurance

           Integrate flow cytometry into routine QC for faster and more reliable data.

  1. Reduce Product Waste and Recalls

          Detect microbial issues early in the production cycle.

  1. Support Regulatory Compliance

          Align with food safety authorities’ expectations in EU, US, and Asia-Pacific regions.

  1. Enable Functional and Probiotic Product Innovation

          Empower your product development team with accurate microbial profiling tools.

  1. Boost Brand Reputation

          Deliver scientifically backed assurance of product quality and health claims.



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